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AAT Bioquest

FAQ

Posted on May 10, 2024
What are the methods I can use to purify nucleic acids?
Antibodies and Proteomics BioconjugationDNA and RNA QuantitationDNA extractionProtein Purification


Posted on May 10, 2024
What is ion exchange chromatography?
Antibodies and ProteomicsAffinity ChromatographyAntibody and Protein LabelingChromatographic techniquesProtein Purification


Posted on May 10, 2024
How does ion exchange chromatography work?
Antibodies and ProteomicsAffinity ChromatographyAntibody and Protein LabelingChromatographic techniquesProtein Purification


Posted on May 10, 2024
What properties of the compounds should I consider when selecting an ion exchange chromatography?
Antibodies and ProteomicsAffinity ChromatographyAntibody and Protein LabelingChromatographic techniquesProtein Purification


Posted on May 10, 2024
What molecules can be purified by ion exchange chromatography?
Antibodies and ProteomicsAffinity ChromatographyAntibody and Protein LabelingChromatographic techniquesProtein Assays & AnalysisProtein Purification


Posted on May 10, 2024
What are the differences between ion exchange chromatography and ion chromatography?
Antibodies and ProteomicsAffinity ChromatographyAntibody and Protein LabelingChromatographic techniquesProtein Assays & AnalysisProtein Purification


Posted on May 10, 2024
What are the differences between anion exchange chromatography and cation exchange chromatography?
Affinity ChromatographyAntibody and Protein LabelingChromatographic techniquesProtein Assays & AnalysisProtein Purification


Posted on May 10, 2024
What are the applications of ion exchange chromatography?
Antibodies and ProteomicsAffinity ChromatographyAntibody and Protein LabelingChromatographic techniquesProtein Assays & AnalysisProtein Purification


Posted on May 10, 2024
How does affinity chromatography work?
Antibodies and ProteomicsAffinity ChromatographyAntibody and Protein LabelingProtein Assays & AnalysisProtein Purificationaffinity purification


Posted on May 8, 2024
What are the applications of Chromatography?
Affinity ChromatographyChromatographic techniquesProtein Purificationaffinity purification


Posted on May 8, 2024
How is the electrophoresis method similar to the chromatographic method?
Affinity ChromatographyChromatographic techniquesProtein Purificationaffinity purification


Posted on May 8, 2024
How do I select the right column for the column chromatography?
Affinity ChromatographyChromatographic techniquesProtein Purificationaffinity purification


Posted on May 8, 2024
How do I prepare column chromatography?
Affinity ChromatographyChromatographic techniquesProtein Purificationaffinity purification


Posted on May 8, 2024
What is specificity in chromatography?
Affinity ChromatographyChromatographic techniquesProtein Purificationaffinity purification


Posted on May 8, 2024
Why does specificity in chromatography matter?
Affinity ChromatographyChromatographic techniquesProtein Purificationaffinity purification


Posted on April 19, 2024
What is diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein Purificationaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the applications of diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein Purificationaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is the process of diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein PurificationSerial Dilutionaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the benefits of diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein PurificationSerial Dilutionaffinity purificationcell lysisdetergent


Posted on August 23, 2023
What are the principles of chromatography?
Affinity ChromatographyChromatographic techniquesProtein Purificationaffinity purification


Posted on April 18, 2023
How do I choose a method for protein purification?
Antibodies and Proteomics BioconjugationAffinity ChromatographyAntibody LabelingAntibody and Protein LabelingChromatographic techniquesProteinProtein Purification


Posted on August 12, 2020
What is protein purification?
Protein PurificationExtractionPrecipitationChromatographic techniques


Posted on June 22, 2020
How do I remove protein tags after purification?
ProteinProtein PurificationPurification


Posted on June 22, 2020
What is a His tag? How does it work?
Antibodies and ProteomicsAntibody and Protein LabelingProteinProtein Purification


Posted on June 22, 2020
What types of protein tags are commonly used?
Peptide and OligonucleotideProteinProtein PurificationPurification


Posted on June 1, 2020
What are theoretical plates in HPLC?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
Why is trifluoroacetic acid (TFA) used in reverse-phase chromatography for protein purification?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is the difference between nitrocellulose and PVDF membranes in western blot?
Antibodies and ProteomicsGel ElectrophoresisProtein PurificationWestern Blot


Posted on June 1, 2020
What kinds of interactions are used in affinity chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is size-exclusion chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What are the exclusion limit and permeation limit in size-exclusion chromatography (SEC)?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is void volume in size exclusion chromatography (SEC)?
Affinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is size-exclusion chromatography (SEC) used for in biochemistry?
Affinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is ion-exchange chromatography (IXC)?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
Why is high concentration of NaCl used in ion-exchange chromatography for elution?
Affinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
How do you do ion exchange chromatography?
Affinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
How do you choose a buffer for ion exchange chromatography?
Affinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is the difference between ion exchange chromatography and affinity chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is protein A chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is the difference between SDS-PAGE and western blot?
Antibodies and ProteomicsGel ElectrophoresisProtein PurificationWestern Blot


Posted on June 1, 2020
Why are proteins transferred from gel to a membrane in western blot?
Gel ElectrophoresisProtein PurificationWestern Blot


Posted on April 24, 2020
How can I increase the expression level of a protein?
Antibodies and ProteomicsAffinity ChromatographyAntibody and Protein LabelingChromatographic techniquesExtractionProteinProtein Purification


Posted on April 24, 2020
What is the difference between BCA and Micro BCA assay?
Antibodies and ProteomicsAffinity ChromatographyBCA assayBradford assayChromatographic techniquesExtractionProteinProtein PurificationProtein concentration